Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite.

Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite.

The stress response is one mechanism that the bladder urothelium could potentially employ to protect itself from cellular damage after exposure to arsenic and, in so doing, influence the shape of the dose-response curve at low concentrations of exposure to this environmental pollutant. In the present study, we used the cultured human urothelial cell line UROtsa, a model of human urothelium, to ...

The human HSP 70 / HSP 40 chaperone family

Hsp 104 , Hsp 70 , and Hsp 40 : A Novel Chaperone System that Rescues Previously Aggregated

are involved in protein turnover. Neither ClpA nor ClpX Hsp104 is a stress tolerance factor that promotes the has intrinsic proteolytic activity. Rather, both proteins reactivation of heat-damaged proteins in yeast by an confer ATP-dependent turnover of their substrates unknown mechanism. Herein, we demonstrate that through a physical association with an unrelated oligoHsp104 functions in this ...

Analysis of hsp 30, hsp 70 and ubiquitin gene expression in Xenopus laevis tadpoles.

Heat-induced accumulation of hsp 30 mRNA (1.1 kb) during early development of Xenopus laevis was first detectable at the tailbud stage (stage 30-34). This contrasts with heat-induced accumulation of hsp 70 mRNA (2.7 kb) and ubiquitin mRNA (size range = 1.7-3.1 kb), which was first detectable at the mid- to late-blastula stage. Continuous exposure of tadpoles to a 33 degrees C heat shock resulte...

Expression of microinjected hsp 70/CAT and hsp 30/CAT chimeric genes in developing Xenopus laevis embryos.

The expression of microinjected chimeric genes containing Drosophila hsp 70 and Xenopus hsp 70 and hsp 30 promoters linked to the reporter gene coding for bacterial chloramphenicol acetyltransferase (CAT) was examined during early development of Xenopus laevis. Heat-inducible expression of fusion genes containing either the Drosophila hsp 70 promoter (1100 bp) or the Xenopus hsp 70 promoter (75...